Journal: International Journal of Nanomedicine

Article Title: Human serum albumin-based probes for molecular targeting of macrophage scavenger receptors

doi: 10.2147/IJN.S197990

Figure Lengend Snippet: Evaluation of Mal-HSA-FITC uptake by different cell types. Fluorescence microscopy images of Mal-HSA-FITC probes (green) incubated for 3 h with ( A ) THP-1 macrophages, ( C ) HUVECs (EC), and ( D ) HCtSMCs (SMC). ( B ) Mal-HSA-FITC uptake by different cell types plotted against time. The results represent data from technical triplicates (9 images/well and timepoint) and are presented as mean and SEM. Data show significantly increased uptake of modified HSA probes by THP-1 cells compared with HUVEC (**** P <0.0001) and HCtSMCs ( #### P<0.0001) at 2.5 h and 3 h. None of the cell types recognized HSA-FITC (data not shown) (repeated measures two-way ANOVA (factors: cell type and time) and Tukey’s multiple comparison test). Abbreviations: HSA, human serum albumin; GCU, green calibration unit.

Article Snippet: Human carotid smooth muscle cells (HCtSMCs) (Cell Applications, San Diego, CA, USA) were cultured up to passage 7 in human smooth muscle cell media (Cell Applications).

Techniques: Fluorescence, Microscopy, Incubation, Modification, Comparison

Journal: Scientific Reports

Article Title: Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor

doi: 10.1038/s41598-021-84036-9

Figure Lengend Snippet: Quantification and comparison of collagen, smooth muscle cells, and interstitial spaces between the murine endo- and ectocervix by using Visiopharm Software on Masson Trichome stained cervical sections. NP, GD15, GD18, GD19, and laboring cervixes were all combined in the analysis using Visiopharm Software Engine (version 6.6.1.2572) to evaluate overall trends in the regions of interest. Data in the bar graphs are presented as mean ± SEM. Proportion of collagen ( A ), smooth muscle cells ( B ), and interstitial spaces ( C ) were calculated as the area of interest (e.g. collagen, SMCs or interstitial space) divided by the total area of the endocervix or ectocervix. Endocervix has a significantly lower amount of collagen, with a significantly higher amount of SMCs than the ectocervix. There is no significant difference in the interstitial spaces between ectocervix and endocervix ( p = 0.31). Statistical significance was determined by t-test. * p < 0.05, ** p < 0.01, *** p < 0.001. (n = 15/region).

Article Snippet: Figure 5 Quantification and comparison of collagen, smooth muscle cells, and interstitial spaces between the murine endo- and ectocervix by using Visiopharm Software on Masson Trichome stained cervical sections.

Techniques: Comparison, Software, Staining

Journal: Scientific Reports

Article Title: Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor

doi: 10.1038/s41598-021-84036-9

Figure Lengend Snippet: Quantification of the smooth muscle area (SMCs), collagen, and interstitial spaces in the murine endo- and ectocervix across normal gestation and term labor by using Visiopharm Software on Masson Trichome stained cervical sections. Cervixes from gestational day (GD) 15, 18, 19 and term labor, were stained by Masson Trichome histologic stain, then underwent image analysis using Visiopharm Software Engine (version 6.6.1.2572). Data is represented as stacked bars, where the length of each portion of the bar (SMCs = dotted, collagen = grey , and interstitial spaces = black ) corresponds to their respective area (mm 2 ) that contributes to entire area of the ( A ) endo- or ( B ) ectocervix at that gestational time point (i.e. the entire length of the bar is the total area of the endo-or ectocervix). Increasing areas of interstitial spaces drive the increase in total cervical area of both sub-regions of mouse cervix. (n = 3–4/GD).

Article Snippet: Figure 5 Quantification and comparison of collagen, smooth muscle cells, and interstitial spaces between the murine endo- and ectocervix by using Visiopharm Software on Masson Trichome stained cervical sections.

Techniques: Software, Staining

Journal: Scientific Reports

Article Title: Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor

doi: 10.1038/s41598-021-84036-9

Figure Lengend Snippet: Heterogeneity of the 3 zones in the murine endocervix using all gestational samples.

Article Snippet: Figure 5 Quantification and comparison of collagen, smooth muscle cells, and interstitial spaces between the murine endo- and ectocervix by using Visiopharm Software on Masson Trichome stained cervical sections.

Techniques: